Shiv Shakti Chemicals

Nutrient media are basic culture media used for maintaining microorganisms, cultivating fastidious organisms by enriching with serum or blood and are also used for purity checking prior to biochemical or serological testing (1,2). Nutrient Broth has the formula originally designed for use in the Standard Method for Examination of Water and Waste water. It is one of the several non-selective media useful in routine cultivation of microorganisms (3,4). It can be used for the cultivation and enumeration of bacteria which are not particularly fastidious. Addition of different biological fluids such as horse or sheep blood, serum, egg yolk etc. makes it suitable for the cultivation of related fastidious organisms. Peptone, HM peptone B and yeast extract provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients necessary for the growth of bacteria. Sodium chloride maintains the osmotic equilibrium of the medium.

Clinical samples - faeces, urine etc.; Food and dairy samples; Water samples.

For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (3,4). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (5). For clinical samples follow appropriate techniques for handling specimens as per established guidelines (6,7). After use, contaminated materials must be sterilized by autoclaving before discarding.

• Gram Stains - Kit
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•  Intended use Grams Stain Kit is used for differentiation of bacteria on the basis of their gram nature. 
• Kit Contents 
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• S012 Gram's crystal violet 125ml 
• S013 Gram’s Iodine 125ml 
• S032 Gram’s Decolourizer 2x125ml 
• S027 Safranin, 0.5% w/v (counter stain) 125ml

Agar powder, Bacteriological Grade is manufactured from species of red seaweeds by observing good manufacturing practice. It is a Bacteriological grade powder with high mineral/metal content and is advantageous to use in certain media. It is recommended for use in bacteriological culture media and plant tissue culture media, where clarity and compatibility are not of prime importance.

• Quality Control
  • Appearance: Cream coloured powder homogeneous free flowing powder
  • Solubility: Freely soluble in hot water at temperatures above 85°C. Insoluble in cold water.
  • Clarity: A firm solid, clear to slightly opalescent gel is formed at a concentration of 1.5% at 38-40°C.
  • Dye Diffusion: Agar dye diffusion :- 18-20mm
  • pH: pH of 1.5% w/v aqueous solution at 25 °C 6.5-7.5